Qualitative Analysis - Problem 9
Advertisements
Description:
Problem − The Rf value of A and B in a mixture determined by TLC in a solvent mixture 0.65 and 0.42. If the mixture was separated using column chromatography using the same solvent, then which one would elute first?
Solution − Given,
Rf(A) = 0.65
Rf(B) = 0.42
Since, A travelled farther away from the solvent base, it was less strongly adsorbed while lower Rf value of B shows that it is more strongly adsorbed by the adsorbent.
Thus, when extracted using column chromatography, A gets eluted first as it is less strongly adsorbed.
Advertisements